Legal Medicine
Volume 5, Issue 1 , Pages 55-59, March 2003

Analysis of the polymorphic structure of short tandem repeats locus D18S555

Department of Legal Medicine, Teikyo University School of Medicine, Kaga 2-11-1, Itabashi-ku, Tokyo 173-8605, Japan

Received 26 August 2002; received in revised form 17 September 2002; accepted 26 September 2002.

Abstract 

We analyzed the polymorphic structure of the short tandem repeats (STR) (RRGG) locus D18S555 by DNA sequencing, and examined the distribution of alleles in DNA samples from Japanese and Chinese individuals. DNA sequence analysis showed that the alleles at this locus consisted of deletions or insertions of (gaaagaaagaat), (ggaa), (ggaaggaaggag), (ggaaggagggaaggaaggag), (gggaggaa), and (ggga) 5, with each allele having its own DNA sequence. Analysis of 200 unrelated Japanese and 169 unrelated Chinese subjects identified 11 alleles, of which alleles 4, 6, and 13 occurred with a high frequency. There was no difference in allele frequency between the two ethnic groups. These results indicate that the genotyping of D18S555 alleles requires no sequence analysis, and making the locus applicable to forensic identity.

Keywords: D18S555-STR locus, Sequence analysis, Polymorphic structure, Japanese population, Chinese population

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PII: S1344-6223(02)00071-8

Legal Medicine
Volume 5, Issue 1 , Pages 55-59, March 2003

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