Legal Medicine
Volume 11, Issue 6 , Pages 257-259 , November 2009

A novel method for the diagnosis of drowning by detection of Aeromonas sobria with PCR method

  • Miwako Aoyagi

      Affiliations

    • Department of Forensic Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, 105-8461 Tokyo, Japan
    • Corresponding Author InformationCorresponding author. Tel.: +81 3 5405 1966; fax: +81 3 3435 7306.
    • ,
  • Kimiharu Iwadate

      Affiliations

    • Department of Forensic Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, 105-8461 Tokyo, Japan
    • ,
  • Kenji Fukui

      Affiliations

    • Department of Forensic Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, 105-8461 Tokyo, Japan
    • ,
  • Shuntaro Abe

      Affiliations

    • Department of Forensic Pathology and Science, Nagasaki University, 1-12-4 Sakamoto, Nagasaki-city, Nagasaki 852-8523, Japan
    • ,
  • Kentaro Sakai

      Affiliations

    • Department of Forensic Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, 105-8461 Tokyo, Japan
    • ,
  • Kyoko Maebashi

      Affiliations

    • Department of Forensic Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, 105-8461 Tokyo, Japan
    • ,
  • Eriko Ochiai

      Affiliations

    • Department of Forensic Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, 105-8461 Tokyo, Japan
    • ,
  • Mihoko Nakamura

      Affiliations

    • Department of Forensic Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, 105-8461 Tokyo, Japan

Received 12 March 2009 ,Revised 7 July 2009 ,Accepted 13 July 2009.

References 

  1. Saukko P, Knight B. Immersion deaths. In: Knight’s forensic pathology. 3rd ed.. London: Arnold; 2004;p. 395–411
  2. Courtice FC, Phipps PJ. The absorption of fluids from the lungs. J Physiol (Lond). 1946;105:186–190
  3. Bajanowski T, Brinkmann B, Stefanec AM, Barckhaus RH, Fechner G. Detection and analysis of tracers in experimental drowning. Int J Legal Med. 1998;111:57–61
  4. Lunetta P, Penttila A, Hallfors G. Scanning and transmission electron microscopical evidence of the capacity of diatoms to penetrate the alveolo-capillary barrier in drowning. Int J Legal Med. 1998;111:229–237
  5. Abe S, Suto M, Nakamura H, Gunji H, Hiraiwa K, Hoshiai G. A novel PCR method for identifying plankton in cases of death by drowning. Med Sci Law. 2003;43:23–30
  6. Kobayashi M, Yamada Y, Zhang WD, Itakura Y, Nagao M, Takatori T. Novel detection of plankton from lung tissue by enzymatic digestion method. Forensic Sci Int. 1993;60:81–90
  7. Ludes B, Quantin S, Coste M, Mangin P. Application of a simple enzymatic digestion method for diatom detection in the diagnosis of drowning in purified corpses by diatom analysis. Int J Legal Med. 1994;107:37–41
  8. Azparren J, de la Rosa I, Sancho M. Biventricular measurement of blood strontium in real cases of drowning. Forensic Sci Int. 1994;69:139–148
  9. Azparren JE, Vallejo G, Reyes E, Herranz A, Sancho M. Study of the diagnostic value of strontium, chloride, haemoglobin and diatoms in immersion cases. Forensic Sci Int. 1998;91:123–132
  10. Azparren JE, Ortega A, Bueno H, Andreu M. Blood strontium concentration related to the length of the agonal period in seawater drowning cases. Forensic Sci Int. 2000;108:51–60
  11. Azparren JE, Fernandez-Rodriguez A, Vallejo G. Diagnosing death by drowning in fresh water using blood strontium as an indicator. Forensic Sci Int. 2003;137:55–59
  12. Aoyagi M, Iwadate K, Matsuyama N, Nara A, Shimada R, Ito Y. An applied diagnosis of drowning by detection of the aquatic bacterium with the PCR method: a case report. Acta Crim Jpn. 2006;72:49–53
  13. Wang G, Clark GC, Liu C, Pucknell C, Munro KC, Kruk MACT, et al. Detection and characterization of hemolysin genes in Aeromonas hydrophila and Aeromonas sobria by multiplex PCR. J Clin Microbiol. 2003;41:1048–1054
  14. Paniagua C, Rivero O, Anguita J, Naharro G. Pathogenicity factors and virulence for rainbow trout (Salmo gairdneri) of motile Aeromonas spp. isolated from a river. J Clin Microbiol. 1990;28:350–355
  15. Daily OP, Joseph SW, Coolbaugh JC, Walker RI, Merrell BR, Rollins DM, et al. Association of Aeromonas sobria with human infection. J Clin Microbiol. 1981;13:769–777
  16. Gelbart SM, Prabhudesai M, Magee SM. A case report: Aeromonas sobria gastroenteritis in an adult. Am J Clin Pathol. 1985;83:389–391
  17. Deutsch SF, Wedzina W. Aermonas sobria-associated left-sided segmental colitis. Am J Gastroenterol. 1997;92:2104–2106

PII: S1344-6223(09)00301-0

doi: 10.1016/j.legalmed.2009.07.003

Legal Medicine
Volume 11, Issue 6 , Pages 257-259 , November 2009

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