Sequence polymorphism of the mitochondrial DNA control region in the population of Vojvodina Province, Serbia

Zgonjanin, Dragana; Veselinović, Igor; Kubat, Milovan; Furač, Ivana; Antov, Mirjana; Lončar, Eva; Tasić, Miloš; Vuković, Radenko; Omorjan, Radovan

doi:10.1016/j.legalmed.2009.10.007

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Volume 12, Issue 2 , Pages 104-107, March 2010

Sequence polymorphism of the mitochondrial DNA control region in the population of Vojvodina Province, Serbia

Dragana Zgonjanin
- Institute of Forensic Medicine, Clinical Center Novi Sad, Hajduk Veljkova 1-7, 21000 Novi Sad, Republic of Serbia
- Corresponding author. Address: Institute of Forensic Medicine, Clinical Center Novi Sad, Hajduk Veljkova 1-7, 21000 Novi Sad, Vojvodina Province, Republic of Serbia. Tel.: +381 21 823 488; fax: +381 21 6624 141.
Igor Veselinović
- Institute of Forensic Medicine, Clinical Center Novi Sad, Hajduk Veljkova 1-7, 21000 Novi Sad, Republic of Serbia
Milovan Kubat
- Department of Forensic Medicine and Criminology, University of Zagreb School of Medicine, Zagreb, Croatia
Ivana Furač
- Department of Forensic Medicine and Criminology, University of Zagreb School of Medicine, Zagreb, Croatia
Mirjana Antov
- University of Novi Sad, Faculty of Technology, Department of Applied Chemistry, 21000 Novi Sad, Bul. Cara Lazara 1, Serbia
Eva Lončar
- University of Novi Sad, Faculty of Technology, Department of Applied Chemistry, 21000 Novi Sad, Bul. Cara Lazara 1, Serbia
Miloš Tasić
- Institute of Forensic Medicine, Clinical Center Novi Sad, Hajduk Veljkova 1-7, 21000 Novi Sad, Republic of Serbia
Radenko Vuković
- Institute of Forensic Medicine, Clinical Center Novi Sad, Hajduk Veljkova 1-7, 21000 Novi Sad, Republic of Serbia
Radovan Omorjan
- University of Novi Sad, Faculty of Technology, Department of Applied Chemistry, 21000 Novi Sad, Bul. Cara Lazara 1, Serbia

Received 29 June 2009; received in revised form 27 September 2009; accepted 27 October 2009. published online 07 December 2009.

Abstract

In order to generate and establish the database for forensic identification purposes in Vojvodina Province (Serbia), the sequence of the hypervariable regions 1 (HV1) and 2 (HV2) of the mtDNA control region were determined in a population of 104 unrelated individuals from Vojvodina Province, using a fluorescent-based capillary electrophoresis sequencing method. A total of 93 different haplotypes were found, of these 83 mtDNA types were unique, nine haplotypes were shared by two individuals and one haplotype by three individuals. The variation of mtDNA HV1 and HV2 regions was confined to 116 nucleotide positions, of which 72 were observed in the HV1 and 44 in the HV2. A statistical estimate of the results for this population showed the genetic diversity of 0.9977 and the random match probability of 1.18%. Haplogroup H was the most common haplogroup (43.3%). Haplogroups observed at intermediate levels included clusters U (13.5%), T (10.6%), J (8.6%) and W (5.8%).

Keywords: Mitochondrial DNA, Hypervariable regions, Sequence polymorphism, Population genetics, Vojvodina Province