Genetic variation of 15 autosomal STR loci in a population sample from Poland

Received 23 January 2010; received in revised form 1 April 2010; accepted 6 May 2010. published online 12 July 2010.

Abstract

Fifteen autosomal STR loci included in AmpFℓSTR® NGM™ kit were analyzed in 154 unrelated individuals from Poland. This multiplex kit enables simultaneous amplification of 10 standard STR loci included in AmpFℓSTR® SGM Plus® kit (D3S1358, vWA, D16S539, D2S1338, D8S1179, D19S433, TH01, FGA, D21S11 and D18S51) and five new mini- and midi-STR loci (D10S1248, D22S1045, D2S441, D1S1656 and D12S391). Population study was conducted to evaluate usefulness of the loci (especially the five new microsatellite systems) in forensic genetic identification examinations. All 15 markers were found to be in Hardy–Weinberg equilibrium. The combined probability of match for the 15 studied STR loci was 3.998×10−19. The same parameter calculated for five new microsatellite loci equaled 8.83×10−7. Discrimination power was particularly high in case of D1S1656 (0.975) and D12S391 (0.972) STR loci.

Keywords: Population data, AmpFℓSTR® NGM™ kit, PowerPlex® ESI 17, MiniSTR, Forensic genetics, Poland

a Institute of Forensic Research, Section of Forensic Genetics, Westerplatte 9, 31-033 Krakow, Poland

b Department of Genetics and Evolution, Institute of Zoology, Jagiellonian University, Ingardena 6, 30-060 Krakow, Poland

Corresponding author at: Department of Genetics and Evolution, Institute of Zoology, Jagiellonian University, Ingardena 6, 30-060 Krakow, Poland.

PII: S1344-6223(10)00067-2

doi:10.1016/j.legalmed.2010.05.002

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