Announcement of Population DataForensic efficiency parameters of the Investigator Argus X-12 kit in women from two Mestizo and seven Amerindian populations from Mexico
Section snippets
Population
We analyzed a total sample of 641 unrelated Mexican females. Considering the previously described population structure of Mexican-Mestizos [1], samples were taken from the states of Tijuana, Monterrey, and Jalisco representing the Northwest region (n = 309), and from the Yucatan Peninsula on behalf of the Southeast region. Similarly, we studied seven Mexican Amerindian groups (n = 332). They were clustered according to genetic and geographic criteria as follows [2]: i) Northwest region, including
DNA analysis
DNA was extracted from blood samples by standard phenol-chloroform method [3], and from blood stains on FTA® classic cards with FTA purification reagent according to the supplier directions (Whatman Inc., Clifton, NJ). The following X-STR loci were PCR-typed by means of the Investigator Argus X-12 kit according to the manufacturer instructions (Qiagen, Hilden, Germany): DXS7132, DXS7423, DXS8378, DXS10074, DXS10079, DXS10101, DXS10103, DXS10134, DXS10135, DXS10146, DXS10148, and HPRTB.
Quality control
Our laboratory participates in the quality control proficiency test annually organized by the Spanish and Portuguese-Speaking Working Group of the International Society for Forensic Genetics (www.gep-isfg.org). In this exercise, we analyzed the Argus X-12 system.
Result and discussion
Allelic frequencies and HWE were computed for each X-STR locus and population region, which is shown in the Supplementary Table S1. These data are useful for likelihood ratio calculations based on X-linked loci performed by different software (described in http://www.chrx-str.org/). In addition, we estimated allele frequencies in Amerindian groups clustered by geographic criteria: Northwest, Center-West, and South (Table S1). These estimates could be helpful for human identification purposes to
Acknowledgments
We thank the technical assistance of Eliana Arteaga-Jiménez and the financial support partially received from Qiagen (Mexico), Logitlab (QFB Fermin Mercado-Payan) and CONACYT (Grant N° 129693 to H-RV and the scholarship to I-CT).
References (12)
- et al.
Collaborative genetic 418 mapping of 12 forensic short tandem repeat (STR) loci on the human X chromosome
Forensic Sci. Int. Genet.
(2012) - et al.
Pre-Hispanic Mesoamerican demography approximates the present-day ancestry of mestizos throughout the territory of Mexico
Am. J. Phys. Anthrop.
(2009) - et al.
Importance of the geographic barriers to promote gene drift and avoid (pre and post-Columbian) gene flow in Mexican Native groups: evidence from forensic STR loci
Am. J. Phys. Anthropol.
(2016) - et al.
Molecular Cloning: A Laboratory Manual Cold Spring Harbor
Laboratory Press
(1989) Tools for Analysis of Population Statistics
(2001)- et al.
Development of a highly polymorphic STR marker for identity testing purposes at the human androgen receptor gene (HUMARA)
J. Forensic Sci.
(1998)
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Allele and haplotype frequencies of 12 X-STRs in Mexican population
2019, Forensic Science International: GeneticsForensic parameters of the X-STR Decaplex system in Mexican populations
2018, Legal MedicineCitation Excerpt :Short Tandem Repeats of the X-chromosome (X-STRs) have demonstrated to be informative in forensic casework [1,2]. However, few X-STR systems have been studied in the Mexican population [3], which is characterized by different levels of admixture originated after the European contact. We studied DNA of 529 female volunteers who signed an informed consent endorsed by the Research Ethical Committee of the local University (OMMITED FOR REVISION PURPOSES).
Demographic processes of the Mexican population from X-STRs included in the Investigator Argus X-12 Kit
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Applications of X-Chromosome Short Tandem Repeats for Human Identification: A Review
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