Forensic efficiency parameters of the Investigator Argus X-12 kit in women from two Mestizo and seven Amerindian populations from Mexico

doi:10.1016/j.legalmed.2017.03.006

Legal Medicine

Volume 26, May 2017, Pages 62-64

https://doi.org/10.1016/j.legalmed.2017.03.006 Get rights and content

Highlights

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Seven Amerindian groups and two Mestizo (admixed) populations from Mexico were studied.
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Allele frequencies and forensic parameters of the ArgusX-12 kit were estimated.
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Hardy-Weinberg equilibrium was observed in practically all loci/populations.
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Genetic relationships and population structure was evaluated.
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Historical records and X-linked inheritance pattern support the observed population structure.

Abstract

Allele frequency distribution and statistical parameters of forensic efficiency concerning the Investigator Argus X-12 kit (Qiagen, Hilden, Germany) were determined in a total sample of 641 unrelated Mexican females, including two Mestizo–admixed– populations (n = 309) and seven Amerindian groups (n = 332) from the main regions of the country. Most of the 12 X-STRs were in agreement with Hardy-Weinberg expectations in all nine Mexican populations. The power of discrimination in females (PD) and Median exclusion chance for trios (MEC_T) and duos (MEC_D) of this genetic system based on X-STRs were >99.99%. Although Mexican populations showed significant pairwise differentiation, a closer relationship was evident between Amerindian groups and nearby Mestizos, in agreement with historical records, previous genetic studies, and X-linked inheritance pattern expectations.

Section snippets

Population

We analyzed a total sample of 641 unrelated Mexican females. Considering the previously described population structure of Mexican-Mestizos [1], samples were taken from the states of Tijuana, Monterrey, and Jalisco representing the Northwest region (n = 309), and from the Yucatan Peninsula on behalf of the Southeast region. Similarly, we studied seven Mexican Amerindian groups (n = 332). They were clustered according to genetic and geographic criteria as follows [2]: i) Northwest region, including

DNA analysis

DNA was extracted from blood samples by standard phenol-chloroform method [3], and from blood stains on FTA® classic cards with FTA purification reagent according to the supplier directions (Whatman Inc., Clifton, NJ). The following X-STR loci were PCR-typed by means of the Investigator Argus X-12 kit according to the manufacturer instructions (Qiagen, Hilden, Germany): DXS7132, DXS7423, DXS8378, DXS10074, DXS10079, DXS10101, DXS10103, DXS10134, DXS10135, DXS10146, DXS10148, and HPRTB.

Quality control

Our laboratory participates in the quality control proficiency test annually organized by the Spanish and Portuguese-Speaking Working Group of the International Society for Forensic Genetics (www.gep-isfg.org). In this exercise, we analyzed the Argus X-12 system.

Result and discussion

Allelic frequencies and HWE were computed for each X-STR locus and population region, which is shown in the Supplementary Table S1. These data are useful for likelihood ratio calculations based on X-linked loci performed by different software (described in http://www.chrx-str.org/). In addition, we estimated allele frequencies in Amerindian groups clustered by geographic criteria: Northwest, Center-West, and South (Table S1). These estimates could be helpful for human identification purposes to

Acknowledgments

We thank the technical assistance of Eliana Arteaga-Jiménez and the financial support partially received from Qiagen (Mexico), Logitlab (QFB Fermin Mercado-Payan) and CONACYT (Grant N° 129693 to H-RV and the scholarship to I-CT).

References (12)

M. Nothnagel et al.
Collaborative genetic 418 mapping of 12 forensic short tandem repeat (STR) loci on the human X chromosome
Forensic Sci. Int. Genet.
(2012)
R. Rubi-Castellanos et al.
Pre-Hispanic Mesoamerican demography approximates the present-day ancestry of mestizos throughout the territory of Mexico
Am. J. Phys. Anthrop.
(2009)
H. Rangel-Villalobos et al.
Importance of the geographic barriers to promote gene drift and avoid (pre and post-Columbian) gene flow in Mexican Native groups: evidence from forensic STR loci
Am. J. Phys. Anthropol.
(2016)
J. Sambrook et al.
Molecular Cloning: A Laboratory Manual Cold Spring Harbor
Laboratory Press
(1989)
A. Tereba
Tools for Analysis of Population Statistics
(2001)
D. Desmarais et al.
Development of a highly polymorphic STR marker for identity testing purposes at the human androgen receptor gene (HUMARA)
J. Forensic Sci.
(1998)

There are more references available in the full text version of this article.

Cited by (6)

Allele and haplotype frequencies of 12 X-STRs in Mexican population
2019, Forensic Science International: Genetics
The use of X-chromosome short tandem repeats (X-STRs) to solve complex kinship cases has been facilitated by commercial human identification kits, such as the Argus X-12 kit (Qiagen), and the free-access software FamlinkX. For this purpose, allele and haplotype frequencies are required in the populations to be employed. Therefore, we obtained Argus X-12 haplotypes in 933 unrelated males from seven different geographic regions from Mexico. Forensic parameters for individual X-STRs and for three-loci linkage groups are reported. The observed homogeneity between the studied population samples support to use a global Mexican population database (Fst p-value >0.05). In brief, forensic validation of the Argus X-12 kit was performed to facilitate incorporation of X-STRs in forensic casework in this country.
Forensic parameters of the X-STR Decaplex system in Mexican populations
2018, Legal Medicine
Citation Excerpt :
Short Tandem Repeats of the X-chromosome (X-STRs) have demonstrated to be informative in forensic casework [1,2]. However, few X-STR systems have been studied in the Mexican population [3], which is characterized by different levels of admixture originated after the European contact. We studied DNA of 529 female volunteers who signed an informed consent endorsed by the Research Ethical Committee of the local University (OMMITED FOR REVISION PURPOSES).
We studied the X-STR decaplex system in 529 DNA female samples of Mexican populations from five geographic regions. Allele frequencies and forensic parameters were estimated in each region and in the pooled Mexican population. Genotype distribution by locus was in agreement with Hardy-Weinberg expectations in each Mexican population sample. Similarly, linkage equilibrium was demonstrated between pair of loci. Pairwise comparisons and genetic distances between Mexican, Iberoamerican and one African populations were estimated and graphically represented. Interestingly, a non-significant interpopulation differentiation was detected (Fst = 0.0021; p = .74389), which allows using a global Mexican database for forensic interpretation of X-STR genotypes.
Demographic processes of the Mexican population from X-STRs included in the Investigator Argus X-12 Kit
2017, Forensic Science International: Genetics Supplement Series
Citation Excerpt :
In this work, we used available genotype dataset of 12 XSTRs (Investigator Argus X-12, Qiagen) from Mestizo and Native American populations from Mexico to make a deeper analysis of their genetic relationships, structure and admixture. We analyzed the genotype dataset for the Argus X-12 of 641 unrelated Mexican females from two Mestizo (n = 309) and seven Native American populations (n = 332) [7]. The Mestizo population sample included two main regions: Northwest (NW; n = 233) and Southeast (SE; n = 76).
We analyzed the genotype dataset for 12 XSTRs in 641 unrelated females from seven Native-American groups and two Mestizo (admixed) populations from the Northwest and Southeast regions of Mexico. Number of alleles, heterocigosity, number effective of population (Ne) were estimated, and some were compared with autosomal and Y-linked genetic systems (aSTRs and YSTRs). Genetic distances were estimated and correlated with geography, discarding the Isolation by distance model (IBD). Geographic and linguistic criteria did not explain the observed population structure with XSTRs in Mexican populations. Among the admixture components, outstanding a peculiar Native American component in Huicholes. In the Northwest, the European ancestry was larger in aSTRs than in XSTRs, but −conversely- in the Southeast the European ancestry is larger in XSTRs than in aSTRs. Although a generalized population expansion was detected in all Mexican Native populations, Bottleneck confirmed significant values in Northwest Mestizos and Nahuas.
Applications of X-Chromosome Short Tandem Repeats for Human Identification: A Review
2023, Journal of Tropical Life Science
Reviews of Science for Science Librarians: Direct-to-Consumer DNA Testing for Ancestry as a Complement to Traditional Genealogical Methods
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Investigation of 12 X-STR loci in Mongolian and Eastern Han populations of China with comparison to other populations
2018, Scientific Reports

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Announcement of Population DataForensic efficiency parameters of the Investigator Argus X-12 kit in women from two Mestizo and seven Amerindian populations from Mexico

Highlights

Abstract

Section snippets

Population

DNA analysis

Quality control

Result and discussion

Acknowledgments

Forensic Sci. Int. Genet.

Pre-Hispanic Mesoamerican demography approximates the present-day ancestry of mestizos throughout the territory of Mexico

Am. J. Phys. Anthrop.

Importance of the geographic barriers to promote gene drift and avoid (pre and post-Columbian) gene flow in Mexican Native groups: evidence from forensic STR loci

Am. J. Phys. Anthropol.

Molecular Cloning: A Laboratory Manual Cold Spring Harbor

Laboratory Press

Tools for Analysis of Population Statistics

Development of a highly polymorphic STR marker for identity testing purposes at the human androgen receptor gene (HUMARA)

J. Forensic Sci.

Announcement of Population Data
Forensic efficiency parameters of the Investigator Argus X-12 kit in women from two Mestizo and seven Amerindian populations from Mexico